APOBEC3A antibody (Internal Region)

Catalog No. ABIN238645

Product Details anti-APOBEC3A Antibody

Target: APOBEC3A (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOBEC3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Phorbolin 1 / APOBEC3A

Sequence: TSNFNNGIGR HKTY

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-TSNFNNGIGRHKTY, from the internal region of the protein sequence according to NP_663745.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx. 26-28 kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0 kDa according to Human NP_663745.1). This molecular weight is routinely observed by other sources. Recommended concent

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APOBEC3A

Target: APOBEC3A (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A))

Alternative Name: APOBEC3A (APOBEC3A Products)

Background: APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1

Gene ID: 200315

NCBI Accession: NP_663745