CD40 antibody (Extracellular Domain) (Biotin)

Catalog No. ABIN2451934

This Mouse Monoclonal antibody specifically detects CD40 in FACS, IHC (fro) and ISt. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for CD40 antibody (Extracellular Domain) (Biotin) (ABIN2451934)

Target: CD40

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunostaining (ISt)

Clone: 5C3

Product Details anti-CD40 Antibody (Biotin)

Binding Specificity: Extracellular Domain

Sterility: Sterile filtered

Immunogen: Recombinant extracellular domain of CD40

Isotype: IgG1 kappa

Application Details

Application Notes: 1. Flowcytometry
2. Immunohistochemistry (acetone-fixed fronzen section,indirect immuno-staining)

Comment:

Conjugate: [Biotin] / [IgG] = 7.1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-CD40 antibody (Biotin) (ABIN2451934)

Ishida, Kumanogoh, Suzuki, Akahani, Noda, Kikutani: "Involvement of CD100, a lymphocyte semaphorin, in the activation of the human immune system via CD72: implications for the regulation of immune and inflammatory responses." in: International immunology, Vol. 15, Issue 8, pp. 1027-34, (2003) (PubMed).

Yasui, Muraoka, Takaoka-Shichijo, Ishida, Takegahara, Uchida, Kumanogoh, Suematsu, Suzuki, Kikutani: "Dissection of B cell differentiation during primary immune responses in mice with altered CD40 signals." in: International immunology, Vol. 14, Issue 3, pp. 319-29, (2002) (PubMed).

Inui, Kaisho, Kikutani, Stamenkovic, Seed, Clark, Kishimoto: "Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40." in: European journal of immunology, Vol. 20, Issue 8, pp. 1747-53, (1990) (PubMed).

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 is a 45-50- kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. This antibody reacts with a 45-48 kDa type I integral membrane glycoprotein present on peripheral blood and tonsillar B cells, but not expressed on terminally differentiated B cells. The antibody against human CD40 was produced from hybridoma (5C3) cultured in serum-free medium and purified under mild conditions by proprietary chromatography processes.

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints