RAD18 antibody (C-Term)

Catalog No. ABIN2452086

Cited in 3+ publications. The Rabbit Polyclonal anti-RAD18 antibody (ABIN2452086) specifically detects RAD18 in WB, ELISA, IP and IF. The antibody is reactive with Human samples.

Quick Overview for RAD18 antibody (C-Term) (ABIN2452086)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-RAD18 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Not reactive to mouse Rad18

Purification: Affinity purified with His-tagged Rad18 protein affinity column

Immunogen: GST-fusion protein containing 113 carboxyl terminal of human Rad18

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 1,000 fold dilution
2) Indirect immunofluorescence staining
3) Immunoprecipitation

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-RAD18 antibody (ABIN2452086)

Watanabe, Tateishi, Kawasuji, Tsurimoto, Inoue, Yamaizumi: "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination." in: The EMBO journal, Vol. 23, Issue 19, pp. 3886-96, (2004) (PubMed).

Miyase, Tateishi, Watanabe, Tomita, Suzuki, Inoue, Yamaizumi: "Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination." in: The Journal of biological chemistry, Vol. 280, Issue 1, pp. 515-24, (2004) (PubMed).

Tateishi, Sakuraba, Masuyama, Inoue, Yamaizumi: "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7927-32, (2000) (PubMed).

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: Rad18

Background: The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 (495 aa, 56 kDa) as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA.