PCDHA12 antibody

Catalog No. ABIN2459278

This Rabbit Polyclonal antibody specifically detects PCDHA12 in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for PCDHA12 antibody (ABIN2459278)

Target: PCDHA12 (Protocadherin alpha 12 (PCDHA12))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCDHA12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PCDHA12 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCDHA12.

Application Details

Application Notes: PCDHA12 antibody can be used for detection of PCDHA12 by ELISA at 1:312500. PCDHA12 antibody can be used for detection of PCDHA12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PCDHA12 antibody at -20 °C.

Target Details for PCDHA12

Target: PCDHA12 (Protocadherin alpha 12 (PCDHA12))

Alternative Name: PCDHA12

Background: PCDHA12 is a potential calcium-dependent cell-adhesion protein. PCDHA12 may be involved in the establishment and maintenance of specific neuronal connections in the brain.This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Molecular Weight: 99 kDa

Gene ID: 56137

NCBI Accession: NP_061726

UniProt: Q9UN75