Protocadherin gamma Subfamily A, 4 (PCDHGA4) antibody

Details for Product No. ABIN2459280
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
4
3
2
1
1
1
1
1
Host
Rabbit
3
1
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
4
Options
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCDHGA4.
Purification Antibody is purified by peptide affinity chromatography method.
Antigen
Alternative Name PCDHGA4 (PCDHGA4 Antibody Abstract)
Background PCDHGA4 is a single-pass type I membrane protein. It contains 6 cadherin domains. PCDHGA4 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain.This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
Molecular Weight 86 kDa
Gene ID 56111
NCBI Accession NP_114442
Application Notes PCDHGA4 antibody can be used for detection of PCDHGA4 by ELISA at 1:62500. PCDHGA4 antibody can be used for detection of PCDHGA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store PCDHGA4 antibody at -20 °C.
Supplier Images
Image no. 1 for anti-Protocadherin gamma Subfamily A, 4 (PCDHGA4) antibody (ABIN2459280) anti-Protocadherin gamma Subfamily A, 4 (PCDHGA4) antibody