ALAS1 antibody

Catalog No. ABIN2459736

This anti-ALAS1 antibody is a Rabbit Polyclonal antibody detecting ALAS1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for ALAS1 antibody (ABIN2459736)

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ALAS1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALAS1.

Application Details

Application Notes: ALAS1 antibody can be used for detection of ALAS1 by ELISA at 1:312500. ALAS1 antibody can be used for detection of ALAS1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALAS1 antibody at -20 °C.

Target Details for ALAS1

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Alternative Name: ALAS1

Background: Delta-aminolevulinate synthase (ALAS, EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2.

Molecular Weight: 70 kDa

Gene ID: 211

NCBI Accession: NP_000679

UniProt: P13196

Pathways: Regulation of Lipid Metabolism by PPARalpha