NFYA antibody

Catalog No. ABIN2460199

This Rabbit Polyclonal antibody specifically detects NFYA in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for NFYA antibody (ABIN2460199)

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NFYA Antibody

Purification: Antibody is supplied as total IgG.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYA.

Application Details

Application Notes: NFYA antibody can be used for detection of NFYA by ELISA at 1:312500. NFYA antibody can be used for detection of NFYA by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 200 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYA antibody at -20 °C.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA

Background: NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

Molecular Weight: 37 kDa, 34 kDa

Gene ID: 4800

NCBI Accession: NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha