SPIB antibody

Catalog No. ABIN2460217

Product Details anti-SPIB Antibody

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPIB.

Application Details

Application Notes: SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SPIB antibody at -20 °C.

Target Details for SPIB

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Alternative Name: SPIB (SPIB Products)

Background: SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1, MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).

Molecular Weight: 19 kDa

Gene ID: 6689

NCBI Accession: NP_003112

UniProt: Q01892