DMAP1 antibody

Catalog No. ABIN2460614

The Rabbit Polyclonal anti-DMAP1 antibody has been validated for WB and ELISA. It is suitable to detect DMAP1 in samples from Human, Mouse and Dog.

Quick Overview for DMAP1 antibody (ABIN2460614)

Target: DMAP1 (DNA Methyltransferase 1-Associated Protein 1 (DMAP1))

Reactivity: Human, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DMAP1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DMAP1.

Application Details

Application Notes: DMAP1 antibody can be used for detection of DMAP1 by ELISA at 1:62500. DMAP1 antibody can be used for detection of DMAP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DMAP1 antibody at -20 °C.

Target Details for DMAP1

Target: DMAP1 (DNA Methyltransferase 1-Associated Protein 1 (DMAP1))

Alternative Name: DMAP1

Background: DMAP1 is a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.

Molecular Weight: 51 kDa, 53 kDa, 53 kDa

Gene ID: 55929

NCBI Accession: NP_001029196

UniProt: Q9NPF5