MTA1 antibody

Catalog No. ABIN2461592

The Rabbit Polyclonal anti-MTA1 antibody has been validated for WB and ELISA. It is suitable to detect MTA1 in samples from Human, Mouse, Rat, Dog and Zebrafish (Danio rerio).

Quick Overview for MTA1 antibody (ABIN2461592)

Target: MTA1 (Metastasis Associated 1 (MTA1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MTA1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MTA1.

Application Details

Application Notes: MTA1 antibody can be used for detection of MTA1 by ELISA at 1:312500. MTA1 antibody can be used for detection of MTA1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MTA1 antibody at -20 °C.

Target Details for MTA1

Target: MTA1 (Metastasis Associated 1 (MTA1))

Alternative Name: MTA1

Background: MTA1 encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. MTA1 was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.

Molecular Weight: 47 kDa, 81 kDa, 59 kDa

Gene ID: 9112

Pathways: Chromatin Binding