MafF antibody

Catalog No. ABIN2461834

This Rabbit Polyclonal antibody specifically detects MafF in ELISA and WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio) and Drosophila melanogaster.

Quick Overview for MafF antibody (ABIN2461834)

Target: MafF (MAFF) (Transcription Factor MafF (MAFF))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MafF antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MafF Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAFF.

Application Details

Application Notes: MAFF antibody can be used for detection of MAFF by ELISA at 1:62500. MAFF antibody can be used for detection of MAFF by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MAFF antibody at -20 °C.

Target Details for MafF

Target: MafF (MAFF) (Transcription Factor MafF (MAFF))

Alternative Name: MAFF

Background: MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. MAFF can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. MAFF may also be involved in the cellular stress response.The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 18 kDa, 18 kDa, 18 kDa, 18 kDa, 18 kDa

Gene ID: 23764

NCBI Accession: NP_690617

UniProt: Q9ULX9

Pathways: Myometrial Relaxation and Contraction