DMAP1 antibody

Catalog No. ABIN2461898

The Rabbit Polyclonal anti-DMAP1 antibody has been validated for WB and ELISA. It is suitable to detect DMAP1 in samples from Human, Mouse, Rat, Dog and Zebrafish (Danio rerio).

Quick Overview for DMAP1 antibody (ABIN2461898)

Target: DMAP1 (DNA Methyltransferase 1-Associated Protein 1 (DMAP1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DMAP1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DMAP1.

Application Details

Application Notes: DMAP1 antibody can be used for detection of DMAP1 by ELISA at 1:1562500. DMAP1 antibody can be used for detection of DMAP1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DMAP1 antibody at -20 °C.

Target Details for DMAP1

Target: DMAP1 (DNA Methyltransferase 1-Associated Protein 1 (DMAP1))

Alternative Name: DMAP1

Background: DMAP1 is a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described.

Molecular Weight: 53 kDa

Gene ID: 55929

NCBI Accession: NP_061973

UniProt: Q9NPF5