Keratin 2 antibody

Catalog No. ABIN2462466

This anti-Keratin 2 antibody is a Rabbit Polyclonal antibody detecting Keratin 2 in WB, IHC and ELISA. Suitable for Human and Dog.

Quick Overview for Keratin 2 antibody (ABIN2462466)

Target: Keratin 2 (KRT2)

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Keratin 2 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRT2A.

Application Details

Application Notes: KRT2A antibody can be used for detection of KRT2A by ELISA at 1:312500. KRT2A antibody can be used for detection of KRT2A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store KRT2A antibody at -20 °C.

Target Details for Keratin 2

Target: Keratin 2 (KRT2)

Alternative Name: KRT2A

Background: KRT2A is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Molecular Weight: 70 kDa

Gene ID: 3849

NCBI Accession: NP_000414

UniProt: P35908