Erythrocyte Ankyrin antibody

Catalog No. ABIN2462605

This Rabbit Polyclonal antibody specifically detects Erythrocyte Ankyrin in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for Erythrocyte Ankyrin antibody (ABIN2462605)

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Erythrocyte Ankyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Erythrocyte Ankyrin Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANK1.

Application Details

Application Notes: ANK1 antibody can be used for detection of ANK1 by ELISA at 1:312500. ANK1 antibody can be used for detection of ANK1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ANK1 antibody at -20 °C.

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: ANK1

Background: Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats, a central region with a highly conserved spectrin binding domain, and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Molecular Weight: 17 kDa, 206 kDa, 15 kDa, 8 kDa

Gene ID: 286

NCBI Accession: NP_065211

UniProt: Q53ER1

Pathways: Synaptic Membrane