MDH1 antibody

Catalog No. ABIN2463166

This Rabbit Polyclonal antibody specifically detects MDH1 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Arabidopsis.

Quick Overview for MDH1 antibody (ABIN2463166)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivity: Human, Mouse, Rat, Arabidopsis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MDH1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MDH1.

Application Details

Application Notes: MDH1 antibody can be used for detection of MDH1 by ELISA at 1:62500. MDH1 antibody can be used for detection of MDH1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MDH1 antibody at -20 °C.

Target Details for MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDH1

Background: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 36 kDa

Gene ID: 4190

NCBI Accession: NP_005908

UniProt: P40925