The Mouse Monoclonal anti-Apolipoprotein M antibody has been validated for WB and ELISA. It is suitable to detect Apolipoprotein M in samples from Human.
APOM
Reactivity: Human
WB, ELISA, IF
Host: Mouse
Monoclonal
2A8
unconjugated
Application Notes
Western Blot:1:500 - 1:2,000 IF:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified antibody in PBS containing 0.03 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage
-20 °C
Storage Comment
ApoM monoclonal antibody can be stored at -20 °C, stable for one year.
Target
Apolipoprotein M (APOM)
Alternative Name
ApoM
Background
ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21 kDa),is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.