Dynamin 1 antibody (truncated)

Catalog No. ABIN2464046

The Mouse Monoclonal anti-Dynamin 1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect Dynamin 1 in samples from Human.

Quick Overview for Dynamin 1 antibody (truncated) (ABIN2464046)

Target: Dynamin 1 (DNM1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3G4B6

Product Details anti-Dynamin 1 Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant Dynamin-1 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2a

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: Dynamin-1 monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin-1

Background: Dynamin-1 (Dyn1), with 864-amino acid protein (about 95 kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.

Gene ID: 1759

UniProt: Q05193

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling