Neurogranin antibody (Internal Region)

Catalog No. ABIN2464335

Product Details anti-Neurogranin Antibody

Target: Neurogranin (NRGN)

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurogranin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Neurogranin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Neurogranin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Neurogranin.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 15 kDa band observed in human brain (cerebral cortex and hippocampus), and in mouse brain and rat brain lysates (calculated MW of 7.6 kDa according to NP_006167.1). There is also an additional 16 kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15,274 (3) :1294-300, PMID: 9880498). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining in the neuronal cell bodies Recommended concentration, 4-6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for Neurogranin

Target: Neurogranin (NRGN)

Alternative Name: Neurogranin (NRGN Products)

Gene ID: 4900

NCBI Accession: NP_006167

UniProt: Q92686