SKIL antibody (AA 600-684)

Catalog No. ABIN2470140

Product Details anti-SKIL Antibody

Target: SKIL (SKI-Like Oncogene (SKIL))

Binding Specificity: AA 600-684

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKIL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Protein G Column

Immunogen: SKIL antibody was raised against amino acids 600 - 684 of SKIL (Human).

Isotype: IgG

Application Details

Application Notes: SKIL antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 2.5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: SKIL antibody can be stored short term 4 °C. For long term storage aliquot and store at -20 °C.

Target Details for SKIL

Target: SKIL (SKI-Like Oncogene (SKIL))

Alternative Name: SKIL (SKIL Products)

Background: Principal Names: SnoN, SNO, SKI-like, Ski-related Oncogene, SnoA, SKIL Official Gene Symbol- SKIL Gen Bank Accession Number- NP_005405 Gene ID- 6498(Human) 20482 (mouse)Gene Map Locus- 3q26 (human) SnoN, an 80 kDa protein, is a member of ski family of nuclear proto-oncogenes involved in regulation of cellular transformation and differentiation. Primarily expressed in two isoforms, SnoN is localized in cytoplasm in normal tissues and non-tumorigenic primary epithelial cells. In cancer tissues or cells, SnoN is exclusively localized in the nucleus. It plays a vital role in inhibition of cell cycle arrest induced by TGF-. Upon morphological differentiation or cell-cycle arrest, SnoN translocates into the nucleus, binds to Smad2, Smad3, and Smad4 on TGF- -responsive promoters and represses their ability to activate expression of TGF- target genes. It has also been found to mediate transcriptional repression of thyroid hormone receptor, Mad and pRb. Increased expression of SnoN has been detected in many human tumor cell lines suggesting a clinical significance.

Gene ID: 6498

UniProt: P12757

Pathways: Positive Regulation of Response to DNA Damage Stimulus