MLF1IP antibody (Thr78)

Catalog No. ABIN2470179

This anti-MLF1IP antibody is a Rabbit Polyclonal antibody detecting MLF1IP in WB, ELISA, IHC and IP. Suitable for Human, Cow, Chimpanzee and Dog.

Quick Overview for MLF1IP antibody (Thr78) (ABIN2470179)

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Reactivity: Human, Cow, Chimpanzee, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1IP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-MLF1IP Antibody

Binding Specificity: Thr78

Specificity: PThr78

Purification: Immunoaffinity Chromatography

Immunogen: MLF1IP antibody was raised against amino acids surrounding Thr78 of MLF1IP (Human).

Application Details

Application Notes: MLF1immunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store MLF1IP antibody at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 °C or below.

Target Details for MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Alternative Name: MLF1IP

Background: Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Gene ID: 79682

UniProt: Q71F23