Ubiquitin antibody (APC)

Catalog No. ABIN2481454

This Rabbit Polyclonal antibody specifically detects Ubiquitin in WB, IHC, ICC, IF and IP. It exhibits reactivity toward Cow.

Quick Overview for Ubiquitin antibody (APC) (ABIN2481454)

Target: Ubiquitin (Ub)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Ubiquitin Antibody (APC)

Specificity: Detects ~10 kDa. It also recognizes ubiquinated proteins.

Cross-Reactivity: Chicken, Cow, Dog, Drosophila melanogaster, Fish, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rainbow Trout, Rat, Saccharomyces cerevisiae, Salmon, Sheep, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogen: Native bovine Ubiquitin, conjugated to KLH

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2481454 was sufficient for detection of free ubiquitin in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).

Gene ID: 281370

NCBI Accession: NP_776558

UniProt: P0CG53

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway