KDEL antibody (PerCP)

Catalog No. ABIN2481797

The Rabbit Polyclonal anti-KDEL antibody has been validated for WB, IHC, ICC and IF. It is suitable to detect KDEL in samples from Mouse, Rat and Human.

Quick Overview for KDEL antibody (PerCP) (ABIN2481797)

Target: KDEL (Lys Asp Glu Leu)

Reactivity: Mouse, Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDEL antibody is conjugated to PerCP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-KDEL Antibody (PerCP)

Specificity: Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.

Purification: Protein A Purified

Immunogen: KDEL containing peptide immunogen

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2481797 was sufficient for detection of KDEL-containing proteins in 20 μg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Target Details for KDEL

Target: KDEL (Lys Asp Glu Leu)

Alternative Name: KDEL

Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.