Piccolo antibody (APC)

Catalog No. ABIN2482380

This Mouse Monoclonal antibody specifically detects Piccolo in WB, ELISA, ICC and IF. It exhibits reactivity toward Mouse.

Quick Overview for Piccolo antibody (APC) (ABIN2482380)

Target: Piccolo (PIC)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Piccolo antibody is conjugated to APC

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 6H9-B6

Product Details anti-Piccolo Antibody (APC)

Specificity: Detects ~550 kDa. No cross-reactivity to Bassoon.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Full length recombinant protein (GenBank ID: BC001304.1)

Isotype: IgG2b kappa

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482380 was sufficient for detection of Piccolo in 20 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Piccolo

Target: Piccolo (PIC)

Alternative Name: Piccolo

Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Gene ID: 26875

NCBI Accession: NP_001104266

UniProt: Q9QYX7