SYT7 antibody (AA 150-239) (Atto 488)

Catalog No. ABIN2482935

This Mouse Monoclonal antibody specifically detects SYT7 in WB, IHC, ICC and IF. It exhibits reactivity toward Mouse.

Quick Overview for SYT7 antibody (AA 150-239) (Atto 488) (ABIN2482935)

Target: SYT7 (Synaptotagmin VII (SYT7))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYT7 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S275-14

Product Details anti-SYT7 Antibody (Atto 488)

Binding Specificity: AA 150-239

Specificity: Detects ~45 kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482935 was sufficient for detection of Synaptotagmin-7 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SYT7

Target: SYT7 (Synaptotagmin VII (SYT7))

Alternative Name: Synaptotagmin 7

Background: Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2.

Gene ID: 54525

NCBI Accession: NP_061271

UniProt: Q9R0N7

Pathways: Synaptic Vesicle Exocytosis