SHANK2 antibody (AA 84-309) (Biotin)

Catalog No. ABIN2484049

The Mouse Monoclonal anti-SHANK2 antibody has been validated for WB, IHC, IP, ICC, IF and AA. It is suitable to detect SHANK2 in samples from Rat.

Quick Overview for SHANK2 antibody (AA 84-309) (Biotin) (ABIN2484049)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Clone: N23b-49 (Formerly S23b-49)

Product Details anti-SHANK2 Antibody (Biotin)

Binding Specificity: AA 84-309

Specificity: Detects ~160 kDa. Recognizes Shank1, 2 and 3.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 84-309 of rat Shank2

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484049 was sufficient for detection of Shank1-4 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: SHANK

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).

Gene ID: 171093

UniProt: Q9QX74