GABBR1 antibody (AA 873-977) (HRP)

Catalog No. ABIN2484102

This Mouse Monoclonal antibody specifically detects GABBR1 in WB, IF and ICC. It exhibits reactivity toward Rat.

Quick Overview for GABBR1 antibody (AA 873-977) (HRP) (ABIN2484102)

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABBR1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S93A-49

Product Details anti-GABBR1 Antibody (HRP)

Binding Specificity: AA 873-977

Specificity: Detects ~115 kDa. No cross-reactivity against GABA(B)R2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484102 was sufficient for detection of GABA(B)R1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GABBR1

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Alternative Name: GABA B Receptor 1

Background: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

Gene ID: 81657

NCBI Accession: NP_112290

UniProt: Q9Z0U4

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process