SLC5A5 antibody (AA 468-643) (Biotin)

Catalog No. ABIN2484440

This anti-SLC5A5 antibody is a Mouse Monoclonal antibody detecting SLC5A5 in WB, IHC and AA. Suitable for Human.

Quick Overview for SLC5A5 antibody (AA 468-643) (Biotin) (ABIN2484440)

Target: SLC5A5 (Solute Carrier Family 5 (Sodium/iodide Cotransporter), Member 5 (SLC5A5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC5A5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Antibody Array (AA)

Clone: 14F

Product Details anti-SLC5A5 Antibody (Biotin)

Binding Specificity: AA 468-643

Specificity: Detects ~97 kDa, non-glycosylated version at 68 kDa. Other minor bands associated with hNIS at 160 kDa, and degradation products at ~30 kDa, and ~15 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Mannose binding protein hNIS fusion (AA468-643)

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484440 was sufficient for detection of hNIS in 20 μg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SLC5A5

Target: SLC5A5 (Solute Carrier Family 5 (Sodium/iodide Cotransporter), Member 5 (SLC5A5))

Alternative Name: Sodium Iodide Symporter

Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).

Gene ID: 6528

NCBI Accession: NP_000444

UniProt: Q92911