TRAP1 antibody (Atto 390)

Catalog No. ABIN2484669

This Mouse Monoclonal antibody specifically detects TRAP1 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for TRAP1 antibody (Atto 390) (ABIN2484669)

Target: TRAP1 (TNF Receptor-Associated Protein 1 (TRAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAP1 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3H4-2H6

Product Details anti-TRAP1 Antibody (Atto 390)

Specificity: Detects ~75 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Purified recombinant TRAP1

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484669 was sufficient for detection of Trap-1/HSP75 in 20 μg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for TRAP1

Target: TRAP1 (TNF Receptor-Associated Protein 1 (TRAP1))

Alternative Name: TRAP1

Background: The 90 kDa heat shock protein (HSP90) family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).

Gene ID: 10131

NCBI Accession: NP_057376

UniProt: Q12931