Rab4 antibody (C-Term) (Atto 488)

Catalog No. ABIN2486630

This anti-Rab4 antibody is a Rabbit Polyclonal antibody detecting Rab4 in WB, IHC, IF and ICC. Suitable for Human.

Quick Overview for Rab4 antibody (C-Term) (Atto 488) (ABIN2486630)

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rab4 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Rab4 Antibody (Atto 488)

Binding Specificity: C-Term

Specificity: Detects ~26 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: C-terminal peptide from human Rab4

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:150)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2486630 was sufficient for detection of Rab4 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Rab4

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Alternative Name: Rab4

Background: Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).

Gene ID: 5867

NCBI Accession: NP_004569

UniProt: P20338