alpha Fetoprotein antibody (AA 68-82)

Catalog No. ABIN2613378

The Goat Polyclonal anti-alpha Fetoprotein antibody has been validated for WB, ELISA and IHC. It is suitable to detect alpha Fetoprotein in samples from Human.

Quick Overview for alpha Fetoprotein antibody (AA 68-82) (ABIN2613378)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-alpha Fetoprotein Antibody

Binding Specificity: AA 68-82

Purpose: alpha-fetoprotein (aa68-82)

Sequence: KDALTAIEKP TGDEQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 68.7 kDa according to NP_001125.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP

Background: AFP, alpha-fetoprotein, FETA, HPAFP, alpha-1-fetoprotein, alpha-fetoglobulin

Gene ID: 174

NCBI Accession: NP_001125

Pathways: C21-Steroid Hormone Metabolic Process