ATP5F1 antibody (AA 142-153)

Catalog No. ABIN2613392

This Goat Polyclonal antibody specifically detects ATP5F1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for ATP5F1 antibody (AA 142-153) (ABIN2613392)

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ATP5F1 Antibody

Binding Specificity: AA 142-153

Purpose: ATP5F1 (aa142-153)

Sequence: SIQHIQNAID TE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 25 kDa band observed in Human Heart lysates (calculated MW of 28.9 kDa according to NP_001679.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1

Background: ATP5F1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1, PIG47, ATP synthase B chain, mitochondrial, ATP synthase subunit b, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1, ATP synthase, H+ transport

Gene ID: 515

NCBI Accession: NP_001679

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process