BCKDHA antibody (C-Term)

Catalog No. ABIN2617503

The Rabbit Polyclonal anti-BCKDHA antibody has been validated for WB, IHC, IF, IHC (p) and ICC. It is suitable to detect BCKDHA in samples from Human.

Quick Overview for BCKDHA antibody (C-Term) (ABIN2617503)

Target: BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDHA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-BCKDHA Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human BCKDHA

Purification: Immunoaffinity purified

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IF (1:100 - 1:1000), IHC, IHC-P (5 μg/mL), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:200 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for BCKDHA

Target: BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Alternative Name: BCKDHA / BCKDE1A

Background: Name/Gene ID: BCKDHA

Synonyms: BCKDHA, BCKDH E1-alpha, BCKDE1A, MSU, MSUD1, OVD1A

Gene ID: 593