DLG1 antibody (Internal Region)

Catalog No. ABIN263164

This Goat Polyclonal antibody specifically detects DLG1 in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for DLG1 antibody (Internal Region) (ABIN263164)

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DLG1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-DLG1 Antibody

Binding Specificity: Internal Region

Purpose: DLG1

Sequence: NSKTRDKGEI PDD

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-NSKTRDKGEIPDD

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 150 kDa in lysate of cell line Kelly after 0.3 μg/mL antibody staining (calculated MW of 103 kDa according to NP_004078.2). This molecular weight is observed by other sources. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and ER of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DLG1

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Alternative Name: DLG1

Background: DLG1 , discs, large homolog 1 (Drosophila) , DKFZp761P0818 , DKFZp781B0426 , DLGH1 , SAP97 , dJ1061C18.1.1 , hdlg, discs large homolog 1 , discs, large homolog 1 , presynaptic protein SAP97 , synapse-associated protein 97

Gene ID: 1739

NCBI Accession: NP_001091894, NP_004078

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response