Cathepsin K antibody (Internal Region)

Catalog No. ABIN263224

This Goat Polyclonal antibody specifically detects Cathepsin K in WB, ELISA and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for Cathepsin K antibody (Internal Region) (ABIN263224)

Target: Cathepsin K (CTSK)

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Cathepsin K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Cathepsin K Antibody

Binding Specificity: Internal Region

Purpose: Cathepsin K

Sequence: KTHRKQYNNK VDE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KTHRKQYNNKVDE, from the internal region (near N Terminus) of the protein sequence according to NP_000387.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Rat Skeletal Muscle lysates and in preliminary testing of Human Skeletal Muscle lysate (calculated MW of 36.8 kDa according to Rat NP_113748.1). Recommended concentration:0.1-1 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the vesicles of U2OSand MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cathepsin K

Target: Cathepsin K (CTSK)

Alternative Name: CTSK

Background: CTSK, cathepsin K, CTS02, CTSO, CTSO1, CTSO2, MGC23107, PKND, PYCD, cathepsin K (pycnodysostosis), cathepsin O1, cathepsin O2, cathepsin X

Gene ID: 1513

NCBI Accession: NP_000387

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades