CD28 antibody (APC)

Catalog No. ABIN264248

The Mouse Monoclonal anti-CD28 antibody has been validated for FACS. It is suitable to detect CD28 in samples from Human.

Quick Overview for CD28 antibody (APC) (ABIN264248)

Target: CD28

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: B-23

Product Details anti-CD28 Antibody (APC)

Specificity: This antibody recats with CD28 receptor.

Characteristics: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Monitoring of activated T cells in peripheral blood. Study of T lymphocyte cytokine function. Study of B cell activation. Study of Cell-adhesion molecules relating T and B lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: Anti-human CD28 binds the 44 kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response