BCL2L1 antibody

Catalog No. ABIN264904

This Sheep Polyclonal antibody specifically detects BCL2L1 in WB. It exhibits reactivity toward Human.

Quick Overview for BCL2L1 antibody (ABIN264904)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BCL2L1 Antibody

Specificity: Reacts with human bcl-xl. The immunogen for the sheep anti-human bcl-xl was a synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence. Overexpression of bcl-xl to other bcl-2 members is believed to promote cell survival. The ratio of bcl-xl to other family members is believed to modulate the apoptotic process.

Purification: Purified

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 - 5 μg/mL, Positive control MCF-7 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: Bcl-2-Like 1

Background: Bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs. The larger mRNA gives rise to a protein product, bcl-xl, which is similar in size and predicted structure to bcl-2 (1). The smaller mRNA gives rise to bcl-xS. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2 however membrane bound forms of bcl-x have been demonstrated in thymocytes (2-4). Following the induction of apoptosis all of the bcl-x protein shifts to the membrane form (2). Of the two isoforms of bcl-x, the long (bcl-xl) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression (1,3). Like its homolog bcl-2, bcl-x undergoes phosphorylation, a modification that requires that a specific 60 amino acid loop region be intact, which in turn appears to regulate activity (5,6). Structurally, based on 3D-structure analysis, bcl-x forms pH sensitive cation-selective ion channels in membranes a property shared with the pore forming domains of several bacterial toxins (7). Bcl-xl has been shown to modify the cell's response to oxidants, to participate in resistance to chemotherapeutic agents and radiation, and to play a key role in the development of the developing CNS (8-10).Synonyms: BCL2L, BCL2L1, BCLX, Bcl-2-like protein 1, Bcl-x, Bcl2-L-1, bcl-xL, bcl-xS

Gene ID: 9606

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling