EDG4 antibody (N-Term)

Catalog No. ABIN265051

This Rabbit Polyclonal antibody specifically detects EDG4 in WB. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for EDG4 antibody (N-Term) (ABIN265051)

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EDG4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EDG4 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes Human EDG-4 / LPA2. Other species not tested.

Purification: Purified

Immunogen: Antibody raised against a unique synthetic peptide derived from the N-terminal of the human EDG4 protein.

Isotype: IgG

Application Details

Application Notes: Western Blot: 5 - 10 μg/mL, should be visualized using a high sensitivitysecondary/substrate system (i. e. Pierce's SuperSignal West Femto Maximum SensitivitySubstrate).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

References for anti-EDG4 antibody (ABIN265051)

Iwai, Koike, Ohshima, Miyatake, Uchiyama, Saeki, Ishii: "RGS18 acts as a negative regulator of osteoclastogenesis by modulating the acid-sensing OGR1/NFAT signaling pathway." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 22, Issue 10, pp. 1612-20, (2007) (PubMed).

Jayapal, Tay, Reghunathan, Zhi, Chow, Rauff, Melendez: "Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses." in: BMC genomics, Vol. 7, pp. 210, (2006) (PubMed).

Shida, Watanabe, Aoki, Hama, Kitayama, Sonoda, Kishi, Yamaguchi, Sasaki, Sako, Konishi, Arai, Nagawa: "Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 10, pp. 1352-62, (2004) (PubMed).

Target Details for EDG4

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Alternative Name: EDG-4 / LPAR2

Background: EDG4 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG4 is lysophospholipid. There are 6 known members of the EDG receptor family and they are implicated in mediating growthrelated effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG4 is expressed in testes, ovarian tumor and leukocyte containing tissues.Synonyms: EDG4, Ipa-2, LPAR2, Lysophosphatidic acid receptor 2, Lysophosphatidic acid receptor Edg-4, endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor 4

Gene ID: 9170

NCBI Accession: NP_004711

UniProt: Q9HBW0