MTA2 antibody (AA 652-668)

Catalog No. ABIN265128

Product Details anti-MTA2 Antibody

Target: MTA2 (Metastasis Associated 1 Family, Member 2 (MTA2))

Binding Specificity: AA 652-668

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts to MTA2 / PID. No cross response to MTA1.

Purification: Purified

Immunogen: Rabbit polyclonal PID antibody was raised against a synthetic peptide corresponding to amino acids 652 to 668 of human PID/MTA2 (6), which differ from the mouse sequence by one amino acid (7).

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 to 1 μg/mL, a 75 kDa band can be detected. Tissue Expression: Widely expressed, located in the nucleus. Positive Control: HeLa cell lysate. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for MTA2

Target: MTA2 (Metastasis Associated 1 Family, Member 2 (MTA2))

Alternative Name: MTA2 (MTA2 Products)

Background: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex (6 - 8). PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis (6). PID/MTA2 is ubiquitously expressed in human tissues (8).Synonyms: MTA1-L1, MTA1L1, Metastasis-associated 1-like 1, Metastasis-associated protein MTA2, PID, p53 target protein in deacetylase complex

Gene ID: 9219

NCBI Accession: NP_004730

UniProt: O94776

Pathways: Chromatin Binding