Netrin 1 antibody

Catalog No. ABIN265183

Product Details anti-Netrin 1 Antibody

Target: Netrin 1 (NTN1)

Reactivity: Human, Mouse, Rat, Monkey, Cow, Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Netrin 1.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide derived from the human Netrin 1 protein.

Isotype: IgG

Application Details

Application Notes: Western blot (0.05-0.5 μg/mL). Positive control: MDCKII cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Can be shipped at 2-8 °C.

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: Netrin-1 (NTN1 Products)

Background: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Netrin 1's main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity.Synonyms: NTN1, NTN1L

Gene ID: 9423

NCBI Accession: NP_004813

UniProt: O95631

Pathways: Regulation of Cell Size