KRT16 antibody

Catalog No. ABIN265495

This Rabbit Polyclonal antibody specifically detects KRT16 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for KRT16 antibody (ABIN265495)

Target: KRT16 (Keratin 16 (KRT16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-KRT16 Antibody

Specificity: This antibody detects endogenous levels of Cytokeratin 16 protein. (region surroundign Ser451)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-KRT16 antibody (ABIN265495)

Yu, Ye, Zhong, Ding, Chen: "Effect of advanced oxidation protein products on articular cartilage and synovium in a rabbit osteoarthritis model." in: Orthopaedic surgery, Vol. 7, Issue 2, pp. 161-7, (2016) (PubMed).

Zhang, Han, Song, Luo, Jin, Mu, Su, Ji, Ren, Liu: "Interferon-? regulates the function of mesenchymal stem cells from oral lichen planus via indoleamine 2,3-dioxygenase activity." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 44, Issue 1, pp. 15-27, (2015) (PubMed).

Suga, Sugimura, Koshiishi, Yorifuji, Makino, Takeda: "Heparin/heparan sulfate/CD44-v3 enhances cell migration in term placenta-derived immortalized human trophoblast cells." in: Biology of reproduction, Vol. 86, Issue 5, pp. 134, 1-8, (2012) (PubMed).

Target Details for KRT16

Target: KRT16 (Keratin 16 (KRT16))

Alternative Name: Cytokeratin 16

Background: Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10 % of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating. Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomasSynonyms: CK16, Cytokeratin-16, K16, KRT16, KRT16A, Keratin 16, Keratin type I cytoskeletal 16, Keratin-16

Molecular Weight: approx. 52 kDa

Gene ID: 3868

NCBI Accession: NP_005548

UniProt: P08779