CD40 antibody (Alexa Fluor 488)

Catalog No. ABIN2656794

This Hamster Monoclonal antibody specifically detects CD40 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD40 antibody (Alexa Fluor 488) (ABIN2656794)

Target: CD40

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to Alexa Fluor 488

Application: Flow Cytometry (FACS)

Clone: HM40-3

Product Details anti-CD40 Antibody (Alexa Fluor 488)

Purification: The antibody was conjugated with Alexa Fluor® 488 under optimal conditions.

Purity: The antibody is at > 85 % purity.

Isotype: IgM kappa, IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints