CD40 antibody (Alexa Fluor 488)

Catalog No. ABIN2657426

This anti-CD40 antibody is a Mouse Monoclonal antibody detecting CD40 in FACS and ICFC. Suitable for Human.

Quick Overview for CD40 antibody (Alexa Fluor 488) (ABIN2657426)

Target: CD40

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to Alexa Fluor 488

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Clone: 5C3

Product Details anti-CD40 Antibody (Alexa Fluor 488)

Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Squirrel Monkey (Saimiri spec.)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints