CCR4 antibody (APC)

Catalog No. ABIN2658514

The Armenian Hamster Monoclonal anti-CCR4 antibody has been validated for FACS and IHC. It is suitable to detect CCR4 in samples from Mouse.

Quick Overview for CCR4 antibody (APC) (ABIN2658514)

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CCR4 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: 2G12

Product Details anti-CCR4 Antibody (APC)

Purification: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CCR4

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Alternative Name: CD194

Background: Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85 % identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naive T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).