CD45RO antibody (APC)

Catalog No. ABIN2658676

The Mouse Monoclonal anti-CD45RO antibody has been validated for FACS, IF, IHC, WB, IP, ICC, Func and ICFC. It is suitable to detect CD45RO in samples from Human.

Quick Overview for CD45RO antibody (APC) (ABIN2658676)

Target: CD45RO

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RO antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func), Intracellular Flow Cytometry (ICFC)

Clone: UCHL1

Product Details anti-CD45RO Antibody (APC)

Cross-Reactivity: Chimpanzee, Marmoset

Purification: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CD45RO

Target: CD45RO

Alternative Name: CD45RO

Background: CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.