2B4 antibody (PE-Cy7)

Catalog No. ABIN2659189

The Mouse Monoclonal anti-2B4 antibody has been validated for ICFC. It is suitable to detect 2B4 in samples from Mouse.

Quick Overview for 2B4 antibody (PE-Cy7) (ABIN2659189)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This 2B4 antibody is conjugated to PE-Cy7

Application: Intracellular Flow Cytometry (ICFC)

Clone: M2B4 (B6)458-1

Product Details anti-2B4 Antibody (PE-Cy7)

Purification: The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244.2

Background: Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.