SSEA-4 antibody (Biotin)
The Mouse Monoclonal anti-SSEA-4 antibody has been validated for FACS. It is suitable to detect SSEA-4 in samples from Human and Mouse.
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Catalog No. ABIN2661286
Quick Overview for SSEA-4 antibody (Biotin) (ABIN2661286)
Target
SSEA-4
(Thiosulfate Sulfurtransferase 4 (SSEA-4))
Reactivity
Human, Mouse
Host
Mouse
Clonality
Monoclonal
Conjugate
This SSEA-4 antibody is conjugated to Biotin
Application
Flow Cytometry (FACS)
Clone
MC-813-70
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Cross-Reactivity
- Mouse (Murine)
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Characteristics
- The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.
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Purification
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
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Isotype
- IgG3 kappa
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Do not freeze.
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Storage
- 4 °C
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Storage Comment
- The antibody solution should be stored undiluted between 2°C and 8°C.
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- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
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Alternative Name
- SSEA-4
Target
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