IL-22 antibody (Ala34) (Biotin)

Catalog No. ABIN2661327

Product Details anti-IL-22 Antibody (Biotin)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Binding Specificity: Ala34

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-22 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Cross-Reactivity (Details): In sandwich ELISA, less than 10 % cross-reactivity with recombinant mouse IL-22.

Purification: The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Immunogen: Ala34-Isoleucin179 (Accession# NM_020525) was expressed in E. coli.

Clone: Poly5161

Isotype: Ig Fraction, IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 1 % BSA and 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL-22 (IL22 Products)

Background: IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79 % amino acid identity with murine IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.