CD77 antibody (FITC)

Catalog No. ABIN2661420

This Mouse Monoclonal antibody specifically detects CD77 in FACS, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for CD77 antibody (FITC) (ABIN2661420)

Target: CD77

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD77 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 5B5

Product Details anti-CD77 Antibody (FITC)

Purification: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CD77

Target: CD77

Background: CD77, also known as Gb3S, A14GALT, A4GALT1, is a type II membrane protein. It was found specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center B lymphocytes. CD77 has been described as a receptor for Shiga Toxin and Verotoxin 1 (VT1), which is produced by Shigella dysenteriae and some strains of E. coli . It is used as a marker for germinal center stage B-cells. CD19 has been reported as a ligand for D77 and their interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.