FOXP3 antibody (Pacific Blue)

Catalog No. ABIN2662095

This Mouse Monoclonal antibody specifically detects FOXP3 in FACS and Func. It exhibits reactivity toward Human.

Quick Overview for FOXP3 antibody (Pacific Blue) (ABIN2662095)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is conjugated to Pacific Blue

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: 259D

Product Details anti-FOXP3 Antibody (Pacific Blue)

Cross-Reactivity: Cynomolgus, Rhesus Monkey, Baboon, Chimpanzee

Purification: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background: FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence, the other (FOXP3 δ2) lacking exon 2. The 259D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation