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ESAM antibody (PE)

The Rat Monoclonal anti-ESAM antibody has been validated for FACS and CyTOF. It is suitable to detect ESAM in samples from Mouse.
Catalog No. ABIN2663690

Quick Overview for ESAM antibody (PE) (ABIN2663690)

Target

See all ESAM Antibodies
ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reactivity

  • 44
  • 25
  • 18
Mouse

Host

  • 60
  • 10
  • 2
Rat

Clonality

  • 62
  • 10
Monoclonal

Conjugate

  • 35
  • 10
  • 10
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ESAM antibody is conjugated to PE

Application

  • 63
  • 25
  • 24
  • 18
  • 13
  • 13
  • 12
  • 6
  • 3
  • 3
  • 3
  • 3
  • 2
Flow Cytometry (FACS), Cytometry by Time of Flight (CyTOF)

Clone

1G8-ESAM
  • Purification

    The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

    Isotype

    IgG2a kappa
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.2 mg/mL

    Buffer

    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from prolonged exposure to light. Do not freeze.

    Storage

    4 °C

    Storage Comment

    The antibody solution should be stored undiluted between 2°C and 8°C.
  • Target

    ESAM (Endothelial Cell Adhesion Molecule (ESAM))

    Alternative Name

    ESAM

    Background

    Endothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation process. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.
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